消减杂交（英語：Subtractive hybridization）这项技术可使对照组（驱赶子）与试验转录物组cDNA片段中仅不相同的部分得到PCR扩增。转录物相对丰度的差异就被突出显示，例如不同物种间的遗传学差异。此项技术基于：对照品与试样间杂交而形成的双链DNA（dsDNA）被移除，因此消除了cDNA或gDNA中丰度相似的部分，仅剩下差异表达或序列、转录物或基因组序列有变异的部分。

抑制消减杂交（英語：suppression subtractive hybridization，简称为SSH）亦成功地被用于鉴定品系或物种间的DNA序列差异，包括弧菌属等多种细菌。

另见

编辑

外部链接

编辑
  • Overview at evrogen.com （页面存档备份，存于互联网档案馆）
  • Munir, Shirin; Singh, Sushmita; Kaur, Kuljeet; Kapur, Vivek. Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells. Biological Procedures Online. 2004-01, 6 (1): 94–104. ISSN 1480-9222. PMC 420231  . PMID 15181476. doi:10.1251/bpo77 （英语）. 
  • Diatchenko, L.; Lau, Y. F.; Campbell, A. P.; Chenchik, A.; Moqadam, F.; Huang, B.; Lukyanov, S.; Lukyanov, K.; Gurskaya, N. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.. Proceedings of the National Academy of Sciences. 1996-06-11, 93 (12): 6025–6030. ISSN 0027-8424. PMC 39182  . PMID 8650213. doi:10.1073/pnas.93.12.6025 （英语）. 
  • Galbraith, Elizabeth A.; Antonopoulos, Dionysios A.; White, Bryan A. Suppressive subtractive hybridization as a tool for identifying genetic diversity in an environmental metagenome: the rumen as a model. Environmental Microbiology. 2004-09, 6 (9): 928–937. ISSN 1462-2912. PMID 15305918. doi:10.1111/j.1462-2920.2004.00575.x （英语）. 


📚 Artikel Terkait di Wikipedia

最小冗余特征选择

Chris Ding and Hanchuan Peng, "Minimum Redundancy Feature Selection from Microarray Gene Expression Data". 2nd IEEE Computer Society Bioinformatics Conference

染色质免疫沉淀-测序

goal and first steps, but does not use cross linking methods and uses microarray instead of sequencing SELEX, a method for finding a consensus binding

子宮內膜

bacteria in the uterus）的存在。 子宮內膜包括一層的柱狀上皮以及柱狀上皮以下的基質（英语：Stroma (animal tissue)）。基質是一層结缔组织，其厚度會依激素的多寡而變化。简单的管状子宫腺從基質的底部延伸到子宮內膜的表面，也有螺旋動脈讓血液可以流通。在女性生殖年

斑馬魚

Cam, M. C. Evaluation of gene expression measurements from commercial microarray platforms. Nucleic Acids Res. (NCBI). 2003, 31 (19): 5676–84. PMC 206463 

他莫昔芬

Identification of estrogen-responsive genes by complementary deoxyribonucleic acid microarray and characterization of a novel early estrogen-induced gene: EEIG1. Molecular

基质辅助激光解吸/电离

spectrometry " validation with triple quadripole tandem mass spectrometry, microarray and PCR. Clin Microbiol Infect. 2014, 20: O1106–12. PMID 24930405. doi:10

肿瘤基因组学

引文格式1维护：显式使用等标签 (link) Lucito R.; et al. , "Representational oligonucleotide microarray analysis: A high-resolution method to detect genome copy number variation