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The selector technique is a method to amplify and multiplex genomic DNA.[1]

Process

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Genomic DNA is digested with restriction enzymes, circularized by hybridisation to selectors and subsequently attached to a vector sequence by ligation. The procedure results in circular DNA molecules with an included general primer pair motif that can be used for amplification by PCR or RCA.

Selector construct

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A selector consists of two oligonucleotides, one Vector oligonucleotide and one Selector probe.[1] Together they form one Selector with target specific ends on each side of a general primer motif.

Selection mechanisms

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  1. A selector probe hybridizes with both ends of the selected target.
  2. A selector probe hybridizes with one end to the 3’ end of the target and the other end to an internal sequence of the target. The protruding 5' end is cleaved off using Taq polymerase.

References

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  1. ^ a b Isaksson, Magnus (December 21, 2010). "Extracting Genomic Variations using Selector Technology". Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine – via www.diva-portal.org.

Publications

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Polymerase chain reaction

COVID-19 testing DNA spiking Loop-mediated isothermal amplification Selector technique Thermus thermophilus Pfu DNA polymerase "The Nobel Prize in Chemistry

Manual transmission

by a selector fork that is linked to the gear lever. The fork does not rotate, so it is attached to a collar bearing on the selector. The selector is typically

Rolling circle replication

proteins, synthesis of metallic nanowires and formation of nano-islands. Selector-technique Ali, M. Monsur; Li, Feng; Zhang, Zhiqing; Zhang, Kaixiang; Kang, Dong-Ku;

CSS

Each rule or rule-set consists of one or more selectors, and a declaration block. In CSS, selectors declare which part of the markup a style applies

Tom Morello

imitate scratching (as on "Bulls on Parade") and working his guitar's pickup selector toggle as a "kill switch" that could be used rhythmically (as on "Know

Agave americana

retrieved 2011-07-28 "RHS Plant Selector - Agave americana 'Marginata'". Retrieved 2015-06-17. "RHS Plant Selector - Agave americana 'Mediopicta'". Retrieved

Polygonatum odoratum

Ehrlén & Eriksson 1991. "RHS Plant Selector – Polygonatum odoratum". Retrieved 28 May 2013. "RHS Plant Selector – Polygonatum odoratum 'Flore Pleno'"

Canna (plant)

Plant Selector – Canna 'Alaska'". Retrieved 19 February 2020. "RHS Plant Selector – Canna 'Annaeei'". Retrieved 19 February 2020. "RHS Plant Selector – Canna